Aspartate Glycosylation Triggers Isomerization to Isoaspartate
نویسندگان
چکیده
منابع مشابه
Chemo-enzymatic detection of protein isoaspartate using protein isoaspartate methyltransferase and hydrazine trapping.
Isoaspartate formation is a ubiquitous post-translation modification arising from spontaneous asparagine deamidation or aspartate isomerization. The formation of isoaspartate inserts a methylene group into the protein backbone, generating a "kink", and may drastically alter protein structure and function, thereby playing critical roles in a myriad of biological processes, human diseases, and pr...
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Although proteins consist exclusively of L-amino acids, we have reported that aspartyl (Asp) 58 and Asp 151 residues of aAcrystallin of eye lenses from elderly cataract donors are highly inverted and isomerized to D-b, D-a and L-b-Asp residues through succinimide intermediates. Of these Asp isomers, large amounts of D-band L-b-isomers are present but the amount of D-a-isomer is not significant....
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2017
ISSN: 0002-7863,1520-5126
DOI: 10.1021/jacs.6b12866